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Expression of miR-22 and PUMA in PC12 cells subjected to I/R. (a) miR-22 was detected by qRT-PCR in PC12 cells subjected to I/R for 0–48 h. (b) Representative Western blot of PUMA in control and I/R groups. (c) The semiquantitative analysis of PUMA protein in PC12 cells at reperfusion 48 h after ischemia period. Data are presented as ±SD. *p < 0.05,**p < 0.01.
