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. 2020 Feb 15;11(1):209–218. doi: 10.1080/21655979.2020.1729321

Figure 2.

Figure 2.

PUMA is target of miR-22. (a) PUMA 3′ UTR contains one predicted miR-22 binding site. The alignment of the seed regions of miR-22 with PUMA 3′ UTR is shown (b) miR-145 mimic transfection inhibits the luciferase activity of PC12 cells transfected with wt-PUMA but not mut-PUMA. (c) anti-miR-22 transfection inhibited miR-22 expression in PC12 cells by qRT-PCR (d) miR-22 downregulation increased endogenous levels of PUMA protein in PC12 cells (e) miR-22 transfection increased miR-22 expression in PC12 cells by qRT-PCR (f) miR-22 upregulation decreases endogenous levels of PUMA protein in PC12 cells. Data are presented as ±SD. **p < 0.01; *p < 0.05.