Figure 5.

COTI-2 treatment inhibits Jurkat xenograft growth (a). Jurkat cells (5 × 10⁶) were injected into the flanks of mice (n = 5 mice per group). Xenografts were allowed to reach 75 ~ 100 mm³ before IP treatment initiation with COTI-2 (10 mg/kg, 5 days a week for 7 weeks) or saline alone. Tumor growth was measured every 5 days by caliper measurement. (b), Tumors were obtained from animals 35 days after drug exposure. Tumors were fixed and stained to examine apopptosis using TUNEL assay. (c), The levels of cleaved-caspase-3 was detected by immunohistochemistry; (d), The levels of miR-203 was detected by qRT-PCR. Significant difference from controls, Student’s t-test, *p < 0.05. **p < 0.01.