Figure 1.

Competitive binding between anti-OVA antibodies. Capture and probe antibodies refer to antibodies in solid phase used to capture OVA and antibodies in solution phase in the assay used to determine cross-competition, respectively. Red and red/black cross-hatched squares indicate no binding of the probe antibody. Green squares indicate binding of the probe antibody. Yellow squares indicate weak binding of probe antibody. OVA-36 and 46, highlighted in black, share IGHV5-25 and IGKV5S2 germline segment use but have distinct binding competition patterns. Groups of squares highlighted with white lines indicate competition of clones with the same V_H/V_L germline segment pairing. Names of antibodies within each V_H/V_L germline segment group are color-coded as in Suppl. Figure 2. Only antibodies with competition data as both capture and probe reagents as well as OVA-36 and 46 are shown, with the full dataset shown in Suppl. Figure 2.