Key resources table.
| Reagent type or resources |
Designation | Source or reference | Identifier | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | colorectal carcinoma | ATCC | HCT116 | Parental HCT116 cell line have been authenticated and all cell lines have been tested negative for mycoplasma. |
| Recombinant DNA reagent | p3xFlag-CMV-14 | Sigma-Aldrich | ||
| Recombinant DNA reagent | pcDNA3.1-MycHis | ThermoFisher | ||
| Antibody | anti-TXNDC11 (Rabbit monoclonal) |
Abcam | Cat#: ab188329 | WB (1:500) |
| Antibody | anti-EDEM2 (Rabbit polyclonal) | Novusbio | Cat#: NBP2-37921 | WB (1:500) |
| Antibody | anti-HA (Rabbit polyclonal) | Recenttec | Cat#: R4-TP1411100 | WB (1:1000) |
| Antibody | anti-calnexin (Rabbit polyclonal) | Enzo Life Sciences | Cat#: ADI-SPA-865 | WB (1:1000) |
| Antibody | anti-PDI (Rabbit polyclonal) | Enzo Life Sciences | Cat#: ADI-SPA-890 | WB (1:1000) |
| Antibody | anti-ERp72 (Rabbit polyclonal) | Enzo Life Sciences | Cat#: ADI-SPA-720 | WB (1:1000) |
| Antibody | anti-calreticulin (Rabbit polyclonal) | Enzo Life Sciences | Cat#: ADI-SPA-600 | WB (1:1000) |
| Antibody | anti-Flag (Mouse monoclonal) | Sigma | Cat#: F3165 | WB (1:1000) IP (2.5 μl) |
| Antibody | anti-β-actin (Mouse monoclonal) | Wako | Cat#: 017–24573 | WB (1:2000) |
| Antibody | Anti-human ATF6α (Rabbit polyclonal) | Haze et al., 1999 | WB (1:1000) |