Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo-sapiens) | Primary human foreskin fibroblasts (HFF) | ATCC | Cat#: CRL-1634 | |
| Strain, strain background (HCMV virus) | TB40E-GFP | O'Connor and Murphy, 2012; Sinzger et al., 2008a | ||
| Antibody | anti-human APC-CD74 (Mouse monoclonal) |
Miltenyi Biotec | Clone: 5–329 RRID:AB_2659190 |
1:200 |
| Antibody | anti-human PE-HLA-DR, DP, DQ (recombinant) |
Miltenyi Biotec | clone: REA332 RRID:AB_2652177 |
1:200 |
| Antibody | anti-human FITC-CD14 (Mouse monoclonal) |
Miltenyi Biotec | Clone: TÜK4 RRID:AB_244303 |
1:200 |
| Antibody | anti-human APC-B7H4 (Mouse monoclonal) |
Biolegend | clone: MIH43 RRID:AB_2562580 |
1:200 |
| Antibody (isotype control) | APC-Mouse IgG1 isotype control (Mouse monoclonal) |
Biolegend | clone: MOPC-21 RRID:AB_326443 |
1:200 |
| Commercial assay or kit | Human CMV HHV5 kit for qPCR using a glycoprotein B target | PrimerDesign | Cat#: Path-HHV5 | |
| Commercial assay or kit | HEX labeled RPP30 copy number assay for ddPCR | Bio-Rad | Cat#: dHsaCP1000485 | |
| Chemical compound, drug | Ruxolitinib | InvivoGen | Cat#: tlrl-rux | 4 µM |
| Peptide, recombinant protein | Human GM-CSF | Peprotech | Cat#: 300-03-20 | 1,000 U/ml |
| Peptide, recombinant protein | Human IL-4 | Peprotech | Cat#: 200-04-20 | 1,000 U/ml |
| Biological compound (E. coli 0111:B4) | LPS | Sigma | Cat#: L4391 | 500 ng/ml |
| Software, algorithm | R 3.5.1 | https://www.r-project.org/ | RRID:SCR_001905 | |
| Software, algorithm | Monocle 2.10.1 | http://cole-trapnell-lab.github.io/monocle-release/ | 2.10.1 | |
| Software, algorithm | DESeq2 1.22.2 | https://bioconductor.org/packages/release/bioc/html/DESeq2.html | RRID:SCR_015687 | |
| Software, algorithm | enrichplot | https://github.com/GuangchuangYu/enrichplot | ||
| Software, algorithm | GSEA 3.0 | http://software.broadinstitute.org/gsea/index.jsp | RRID:SCR_003199 | |
| Software, algorithm | MSigDB 6.2 | http://software.broadinstitute.org/gsea/index.jsp | RRID:SCR_016863 | Database used for GSEA |
| Software, algorithm | CellRanger 2.0.0 | https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger | RRID:SCR_017344 | |
| Software, algorithm | lme4 1.1–21 | https://cran.r-project.org/web/packages/lme4/index.html | RRID:SCR_015654 | R package |
| Software, algorithm | Bowtie2 2.2.9 | http://bowtie-bio.sourceforge.net/bowtie2/index.shtml | ||
| Software, algorithm |
Rtsne 0.15 | https://cran.r-project.org/web/packages/Rtsne/index.html | RRID:SCR_016342 | R package |