Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Saccharomyces cerevisiae) | Yeast strain BY4741 | (Baker Brachmann et al., 1998) | ||
| Antibody | Anti-Histone H3 (mono-methyl K4) polyclonal | Abcam | Abcam Cat# ab8895, RRID:AB_306847 | WB (1:500) |
| Antibody | Anti-Histone H3 (di-methyl K4) polyclonal | Abcam | Abcam Cat# ab7766, RRID:AB_2560996 | WB (1:500) |
| Antibody | Anti-Histone H3 (tri-methyl K4) polyclonal | Abcam | Abcam Cat# ab8580, RRID:AB_306649 | WB (1:500) |
| Antibody | Anti-GAPDH monoclonal | Abcam | Abcam Cat# ab125247, RRID:AB_11129118 | WB (1:2000) |
| Recombinant DNA reagent | pEW106 | This Study | pQE-81L: H3 K4M | |
| Recombinant DNA reagent | pEW66 | This Study | COMPASS expression plasmid pBIG1a containing Bre2, Swd1, Swd2, Sdc1 and Sgh1 | |
| Recombinant DNA reagent | pEW107 | This Study | COMPASS expression plasmid pBIG1b containing 6xHis-3xFLAG-Set1(762–1080), Swd3 and twin-strep-Spp1. | |
| Recombinant DNA reagent | pEW108 | This Study | COMPASS expression plasmid pBIG1ab containing all COMPASS subunits. | |
| Recombinant DNA reagent | WT Set1 | This Study | pEW111 pRS415: WT Set1 | |
| Recombinant DNA reagent | WT Swd1 | This Study | pEW113 pRS415: WT Swd1 |
|
| Recombinant DNA reagent | Set1(R936A) | This Study | pEW118 pRS415: Set1(R936A) |
|
| Recombinant DNA reagent | Set1(R936E) | This Study | pEW120 pRS415: Set1(R936E) |
|
| Recombinant DNA reagent | Set1(R901A) | This Study | pEW123 pRS415: Set1(R901A) |
|
| Recombinant DNA reagent | Set1(R904A) | This Study | pEW124 pRS415: Set1(R904A) |
|
| Recombinant DNA reagent | Set1(R908A) | This Study | pEW125 pRS415: Set1(R908A) |
|
| Recombinant DNA reagent | Set1(R909A) | This Study | pEW126 pRS415: Set1(R909A) |
|
| Recombinant DNA reagent | Set1(R901A, R904A, R908A, R909A) | This Study | pEW127 pRS415: Set1(R901A, R904A, R908A, R909A) |
|
| Recombinant DNA reagent | Set1(R901E) | This Study | pEW128 pRS415: Set1(R901E) |
|
| Recombinant DNA reagent | Set1(R904E) | This Study | pEW129 pRS415: Set1(R904E) |
|
| Recombinant DNA reagent | Set1(R908E) | This Study | pEW130 pRS415: Set1(R908E) |
|
| Recombinant DNA reagent | Set1(R909E) | This Study | pEW131 pRS415: Set1(R909E) |
|
| Recombinant DNA reagent | Set1(R901E, R904E, R908E, R909E) | This Study | pEW132 pRS415: Set1(R901E, R904E, R908E, R909E) |
|
| Recombinant DNA reagent | Swd1(L12A) | This Study | pEW139 pRS415: Swd1(L12A) |
|
| Recombinant DNA reagent | Swd1(E14R) | This Study | pEW140 pRS415: Swd1(E14R) |
|
| Recombinant DNA reagent | Swd1(E14A) | This Study | pEW141 pRS415: Swd1(E14A) |
|
| Recombinant DNA reagent | Swd1(F9A, L12A, E14A) | This Study | pEW142 pRS415: Swd1(F9A, L12A, E14A) |
|
| Recombinant DNA reagent | Swd1(E397R) | This Study | pEW144 pRS415: Swd1(E397R) |
|
| Recombinant DNA reagent | Swd1(V263A) | This Study | pEW145 pRS415: Swd1(V263A) |
|
| Recombinant DNA reagent | Swd1(I264A) | This Study | pEW146 pRS415: Swd1(I264A) |
|
| Recombinant DNA reagent | Swd1(N265A) | This Study | pEW147 pRS415: Swd1(N265A) |
|
| Recombinant DNA reagent | Swd1(K266A) | This Study | pEW148 pRS415: Swd1(K266A) |
|
| Commercial assay or kit | MTase-Glo | Promega | V7601 |