| targeted construct into the act5 locus | plasmid name | number of occupied wells | number of checked clones | Positive clones | Correct clones (%) | Dictyostelium strain used | transfection efficiency (transfectants/ 2x10^6/1 µg DNA) |
| LifeAct-mCherry | pPI226 | 7 | 7 | 1 | 14.2 | AX2 | 3.5x10^-6 |
| LifeAct-GFP | pPI227 | 12 | 12 | 5 | 41.6 | AX2 | 6x10^-6 |
| GFP | pDM1513 | 3 | 3 | 2 | 66.6 | AX2 | 1.5x10^-6 |
| mCherry | pDM1514 | 3 | 3 | 1 | 33.3 | AX2 | 1.5x10^-6 |
| GFP | pDM1513 | 66 | 9 | 5 | 55.5 | AX2 | 3.3x10^-5 |
| mCherry | pDM1514 | 221 | 12 | 10 | 83.3 | AX2 | 1.1x10^-4 |
| H2B-mCherry | pPI420 | 3 | 3 | 1 | 33.3 | AX2 | 1.5x10^-6 |
| H2B-mCherry | pPI420 | 7 | 7 | 6 | 85.7 | AX2 | 3.5x10^-6 |
| mCherry | pDM1514 | 10 | 10 | 7 | 70 | DdB | 5x10^-6 |
| mCherry | pDM1514 | 240 | 12 | 11 | 91.6 | DdB | 1.2x10^-4 |
| mCherry | pDM1514 | 320 | 12 | 12 | 100 | NC4 | 1.6x10^-4 |
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