Extended Data Fig. 10. The scale of genome synthesis, and scale and fidelity of recoding.
a, Genome and chromosome synthesis. The size (in Mb) of synthetic genomes that have been produced for M. genitalium and M. mycoides22,23, and several S. cerevisiae chromosomes24–31 (light grey). The size of the synthetic E. coli genome presented here is shown in dark grey.
b, Genome recoding efforts. Attempts to recode target codons TTA and TTG in Salmonella enterica serovar Typhimurium LT218; AGC, AGT, TTG, TTA, AGA, AGG and TAG in E. coli19; AGA and AGG in E. coli16, as well as recoding of all TAG in E. coli14 (light grey), compared to the removal of all TCA, TCG and TAG in E. coli presented here (dark grey). The total number of codons recoded in a single strain is shown on the graph, and the maximum percentage of target codons recoded in a single strain in each effort is indicated.
c, Number of reported non-programmed mutations and indels as a function of the number of target codons recoded for the experiments shown in b.