A, Basal relative mRNA level of fibronectin was higher in Pre-HT SS rats compared with Pre-HT SSEts1+/− rats. Fibronectin mRNA level further increased in the HT SS rats, and was significantly higher than that observed in the microvessels of HT SSEts1+/− rats (*p-value <0.05 vs Pre-HT SS, Pre-HT SSEts1+/−, and HT SSEts1+/− groups; n=6 animals in each group). B, Immunofluorescence microscopy using alpha-smooth muscle actin (SMA) antibody (Green) to outline the afferent arteriole and antibody to fibronectin (Red) showed deposition of fibronectin around the kidney microvessels of HT SS rats. G, glomerulus.