Table 1.
Primers for Real-time PCR analysis.
| Gene | Forward primer | Reverse primer |
|---|---|---|
| Ets1* | AGGCATTGTGGGTAATAACA | AAGTCCACTGTCGCTGTCTC |
| Ccl2 | CAGGTGTCCCAAAGAAG | CAAAGGTGCTGAAGTCC |
| Fibronectin | GCCTTCAACTTCTCCTGTGA | GTTGCAAACCTTCAATGGTC |
| VCAM | GGGGATTCCGTTGTTCT | CAGGGCTCAGCGTCAGT |
| ICAM | CAAACGGGAGATGAATGG | TGGCGGTAATAGGTGTAAAT |
| P-selectin | AATGAAATCGCTCACCTC | TTATTGGGCTCGTTGTCT |
| Tgfb1 | CTACTGCTTCAGCTCCACAGAGA | ACCTTGGGCTTGCGACC |
| Collagen I | CCAGCCGCAAAGAGTCTACA | AGGCCATTGTGTATGCAGCT |
| Osteopontin | AGGAGTTTCCCTGTTTCTG | GTCTTCCCGTTGCTGTC |
| GAPDH | ATTCTTCCACCTTTGATGC | TGGTCCAGGGTTTCTTACT |
*
Of note, the primers used to amplify Ets1 mRNA also amplify the mRNA of the mutant gene.