Figure 2.

Extracellular and intracellular recording from hiPSC-derived cardiomyocytes on nanoporous TiN electrodes from Multi Channel System MEA. (A) Optical image of a MCS-MEA covered by a monolayer of cardiomyocytes (image taken from the bottom with an inverted microscope). The inset is an optical image acquired from above through the optics used for laser poration; it shows the laser excitation on the TiN electrode during poration. (B) Spontaneous extracellular field potential recording of cardiomyocytes. (C) Detailed view of the instantaneous transition from an extracellular to intracellular AP of cardiomyocytes after optoacoustic poration. (D) Magnification of an intracellular AP of cardiomyocytes. (E) Sequential optoacoustic poration of cardiomyocytes on six different TiN electrodes of the same MCS-MEA.