Fig. 6.

Establishment of a cutoff for abnormal HER2 signaling in the CELx HSF Test. a Three-component normal mixture fit superimposed on a histogram of the individual CELx HSF scores (x axis) for HER2-negative tumors (n = 114). Green, red, and blue curves represent subsets A, B, and C (respectively) of the three components analysis detailed in Table 1. b Inferred ROC curve for component three vs. a composite of components one and two. c Relationships of sensitivity (Sens, false negatives, < 15%) and false positives (FP = 0) to potential CELx HSF Test cutpoints derived from the ROC analysis. The vertical line is the selected cutpoint at 250 CELx Test signaling units