Fig. 2.

Endometrial cell populations can be identified based on their differential expression of cytoskeletal proteins. Relative keratin-18 and vimentin mRNA gene expression in (a) endometrial epithelial and (b) stromal cell populations. Immunofluorescence staining of (c, e) endometrial epithelial and (d, f) stromal fibroblast cells with antibodies against cytoskeletal proteins cytokeratin and vimentin. Scale bar indicates 100 μm. Western blotting staining of (g) endometrial epithelial and (h) stromal fibroblast cell lysates with antibodies against cytokeratin and vimentin. Gel electrophoresis image of PCR products for the PTPRC gene (which encodes CD45 protein) and RPS9 (reference gene) from (i) endometrial epithelial and (j) stromal fibroblast cells. cDNA from PBMCs was included as a positive control