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. 2020 Feb 24;10:3300. doi: 10.1038/s41598-020-60242-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NMR Structure of the AeOBP22-AA Complex (a) A slice through a ¹²C-edited/¹³C-filtered intermolecular NOESY spectrum at δ¹³C = 22.7 ppm showing NOEs between resonances from the protein (labeled in red) and arachidonic acid (black labels). For the lipid the hydrogens are numbered according to the attached carbon in the alkyl chain. (b) Superposition of the 30 lowest energy structures from the final iteration of NMR calculations (RMSD = 0.32 Å for res 7–121), helices 1–7 are color coded from red-violet. The arachidonic acid is shown in cyan.