Figure 4.

Overview of laboratory processes for the two capture kits. For SSFE physical fragmentation is followed by DNA fragment repair and ligation of adapter sequences; hybridisation of patient DNA with the baits and pulldown is then performed, followed by library indexing and pooling¹⁹. For TSO, combined fragmentation and adapter ligation is performed enzymatically, followed by sample amplification, indexing and pooling. Two iterations of bait hybridisation and pulldown are then performed, with a final pooled library amplification¹⁸.