Table 1.
SSFE | TSO | ||||
---|---|---|---|---|---|
Mean | CVa (%) | Mean | CV (%) | ||
Read generated | 86,509,698 | 8.1 | 80,712,320 | 17.7 | |
Reads mapped | 84,816,034 | 8.0 | 74,590,089 | 18.0 | |
Duplicate reads | 21,918,522 | 14.0 | 19,674,723 | 24.0 | |
Reads mapped to target | 46,466,309 | 5.9 | 38,474,359 | 15.6 | |
Reads mapped to target ± 150 bp | 61,283,075 | 6.0 | 48,581,312 | 15.9 | |
Mean coverage of target | 211 | 5.8 | 317 | 15.2 | |
Targetb size (bp) | 17,846,036 | — | 11,946,514 | — | |
Targetb covered (%) with read depth≥: | 1 X | 100 | 0.1 | 99 | |
5 X | 100 | 0.1 | 99 | 0.1 | |
10 X | 99 | 0.1 | 98 | 0.2 | |
20 X | 98 | 0.3 | 98 | 0.3 | |
30 X | 97 | 0.5 | 97 | 0.4 | |
50 X | 92 | 1.1 | 96 | 0.8 | |
100 X | 76 | 2.8 | 91 | 3.0 |
aCoefficient of variation.
bTarget regions as defined by vendor target BED file.