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. 2020 Feb 24;10:3235. doi: 10.1038/s41598-020-60215-y

Table 1.

Summary coverage statistics for triplicate sequenced samples.

SSFE TSO
Mean CVa (%) Mean CV (%)
Read generated 86,509,698 8.1 80,712,320 17.7
Reads mapped 84,816,034 8.0 74,590,089 18.0
Duplicate reads 21,918,522 14.0 19,674,723 24.0
Reads mapped to target 46,466,309 5.9 38,474,359 15.6
Reads mapped to target ± 150 bp 61,283,075 6.0 48,581,312 15.9
Mean coverage of target 211 5.8 317 15.2
Targetb size (bp) 17,846,036 11,946,514
Targetb covered (%) with read depth≥: 1 X 100 0.1 99
5 X 100 0.1 99 0.1
10 X 99 0.1 98 0.2
20 X 98 0.3 98 0.3
30 X 97 0.5 97 0.4
50 X 92 1.1 96 0.8
100 X 76 2.8 91 3.0

aCoefficient of variation.

bTarget regions as defined by vendor target BED file.