Figure 6.

Neonatal mice demonstrate polarized migration of CD103⁺ and CD11b⁺ DC subsets to the mediastinal lymph nodes (mLNs) after intranasal administration of flagellin. (A) Overview of Intranasal administration of flagellin-adjuvanted DQ-OVA and isolation of draining lymph nodes at 24 h. (B) Gating strategy for identification of migratory DC1s and DC2s in draining lymph nodes. (C) Flagellin significantly increases the percentage of OVA-specific CD11b⁺ cells in mLNs of adult mice, but not CD103⁺ DCs. Flagellin significantly increases the percentage of both OVA-specific CD103⁺ and CD11b⁺ DC subsets in neonates. (D,E) Flagellin upregulates expression of CD80 and CD86 on neonatal CD103⁺ migDCs DCs isolated from mLNs. Results are expressed as mean ± SEM of n = 5 per age group. **p < 0.01, ***p < 0.001, ns, not significant, determined by repeated measures two-way ANOVA with Sidak post-hoc test.