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. 2020 Feb 24;10:3303. doi: 10.1038/s41598-020-60158-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of sensor coatings by the Epic BT. (a,b) Sensor responses after the coating with gelatin (a 0.2 mg/ml. b 5 mg/ml). Breaks represent 30 min incubation time outside the instrument. Black: washing two times with PBS, Red: replacement of the solution with PBS, Blue: no further treatment. (c) Typical cell adhesion curves obtained on gelatin (0.2 mg/ml, 5 mg/ml) coatings after the washing step. At 15 min, 20,000 HeLa cells were pipetted into the wells of the sensor plate in 20 μl HEPES HBSS buffer, and recorded for 145 min.