Figure 5.

Fine-tuning of the label-free measurement of dynamic mass redistribution on confluent HUVEC monolayers. A 384 well biosensor microplate was precoated with 0.2 mg/ml gelatin (37 °C, 20 min, PBS), then wells were washed with PBS and confluent monolayers of HUVECs were created on the surface (24 h). The microplate was placed into the Epic BT reader, and after the stabilization of the baseline, cell treatments were added directly to the culture medium, and the wavelength shift was monitored for 60 min. (a) The culture medium was added alone. (b) 2 μM of trypsin was added. (c) 300 nM of thrombin was added. (d) Various concentrations of thrombin were added. The response curves were normalized to the medium control and the maximum values of the wavelength shift were determined. The figures show representative graphs of at least 3 independent measurements.