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. 2020 Feb 24;4:3. doi: 10.1038/s41698-019-0107-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Eluting, pushing with oil, and drying steps were optimized for the PIBEX application in a microfluidic chip. a Membrane size effect on recovery volume of eluent (bar chart) and DNA recovery rate (dotted line) in the PIBEX method were investigated (n = 3). The target initial volume of eluent was 50 μL. The numbers in parentheses denote the percentage of eluent recovery. b Recovery amount of eluent were investigated in centrifugation, vacuum only, and PIBEX chip methods (n = 3). Initial eluent volume varies from 50–150 μL. c Residual ethanol remaining inside silica membrane was monitored in pressure-driven air flow (n = 3). The error bars indicate standard deviation.