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. 2020 Feb 24;4:3. doi: 10.1038/s41698-019-0107-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — The performance of cfDNA extraction in the PIBEX chip compared to the commercial product QIAamp. a DNA recovery rates according to input of fragment DNA was investigated in three experiments. b Purity of final eluents was compared by absorbance ratio at A260/280 (n = 5). c Relative efficiency of cfDNA extraction in healthy plasma sample was measured by real-time PCR with the four reference genes: TERT, NAGK, RPPH1, and GAPDH. The relative efficiency was calculated based on cfDNA extraction in QIAamp (n = 8). d Size analysis of extracted DNA from human plasma of healthy control was measured by microelectrophoresis. The error bars indicate standard deviation.