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. 2020 Feb 24;10:3245. doi: 10.1038/s41598-020-60195-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dasatinib promotes type 1 iNKT cells in mice in vivo. (A–C) Flow cytometry analysis of thymic cells from BALB/c WT mice orally gaved with dasatinib (Dasa, n = 12) or its excipient (CTR, n = 8) for 8 weeks. Analysis of iNKT cell frequency (A) and CD69 positive iNKT cell frequency (B). (C) iNKT cell differentiation into NKT1, NKT2 and NKT17 subtypes: frequency (upper panel) and T-bet, PLZF and RORγt MFI in iNKT cells (lower panel) are shown. Representative plots are shown. Statistical analysis: Mann-Whitney two-tailed.