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. 2020 Feb 18;13:19. doi: 10.3389/fnmol.2020.00019

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Copyright © 2020 Ormeño, Hormazabal, Moreno, Riquelme, Rios, Criollo, Albornoz, Alfaro and Budini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Wild type and aggregated-prone TDP-43 forms are present in lysosomal fractions isolated from cell culture. (A) The lysosomal fraction was isolated from cell line over-expressing Flag-TDP-43 WT during 72 h, then endogenous, as well as Flag-tagged protein was evaluated by Western blot. (B) The lysosomal fraction was isolated from cell line over-expressing Flag-TDP-12xQ/N during 72 h, then endogenous, as well as Flag-tagged protein was evaluated by Western blot. PSN, post-nuclear fraction; F1, post-nuclear fraction after 1st ultracentrifugation step; Mit/Lys, fraction containing mitochondria and lysosomes after 1st ultracentrifugation step; Mit, mitochondria fraction after 2nd centrifugation step; Lys, lysosomal fraction after 2nd centrifugation step. Western blots are representative from at least three independent experiments.