Figure 5.

Aggregated-prone form of TDP-43 co-precipitates with Hsc70. (A) The HEK293 Flp-in, HEK293 Flag-TDP-43 WT and HEK293 Flag-TDP-12xQ/N cell lines were incubated with tetracycline for 72 h. Then, immunoprecipitation was performed using an Anti-Flag M2 Affinity Gel. Immunoprecipitated Flag-tagged proteins were analyzed by Western blot using an anti-Flag M2 antibody. (B) The presence of co-precipitated Hsc70 protein in samples shown in (A) was evaluated by Western blot using an anti-Hsc70 antibody. IN, inputs (20 μg of cell lysate); IP, immunoprecipitates. Western blots are representative from at least three independent experiments.