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. 2020 Feb 24;10:3294. doi: 10.1038/s41598-020-60319-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Binding of murine PBD-C06 to Aβ monomers. Sensograms of human pE3-Aβ(3–40) monomers binding to murine PBD-C06 were obtained with a Biacore 3000 SPR instrument. Binding was determined immediately after monomer preparation, using a flow rate of 30 µl/min and injecting 240 µl of 1 nM (light magenta) to 100 nM (green) of pE3-Aβ(3–40). The sensograms were recorded for 2500 sec and association and dissociation rates were obtained using the 1:1 Langmuir binding model. Resp.Diff. = Difference in Response Units.