Figure 3.

Let-7f affects BM-MSCs proliferation and apoptosis in vitro. (A,B) Apoptotic status of normal non-tranfected BM-MSCs (Control), normal BM-MSCs tranfected with let-7f scrambled oligonucleotides (Scrambled), Let-7-mimics, or Let-7-inhibitor with or without the presence of recombinant human IL-6 (50 ng/ml) or anti-human IL-6 antibody (30 μg/ml) by annexin V and 7-AAD staining assay. Dot plots showing the frequency of Annexin V⁺7-AAD⁺ late apoptotic cells of BM-MSCs among different groups. (C,D) Proliferation status of BM-MSCs among the six groups by CFSE assay. All data are mean ± SEM. n = 6, *P < 0.05, **P < 0.01.