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. 2020 Feb 18;14:126. doi: 10.3389/fnins.2020.00126

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Copyright © 2020 Ueha, Ueha, Kondo, Nishijima and Yamasoba.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Serum immunoglobin E (IgE) levels of the control and cigarette smoke solution (CSS)-treated mice were determined by enzyme linked immunosorbent assay. (B) Representative images of Sirius red staining for eosinophils and periodic acid-Schiff and Alcian blue (PAS/AB) staining for goblet cells in the nasal RM of the control mice and mice treated with 10 doses of CSS (CSS 10). (C) Representative immunohistochemical images of neutrophils and Ki67⁺ dividing cells in the nasal RM (400× magnification), and comparative charts of Ki67⁺ cell counts (n = 6, Mann–Whitney U test). (D) Representative images of olfactory marker protein (OMP)⁺ mature olfactory receptor neurons (ORNs) in two different areas of the olfactory epithelium: the nasal septum and upper lateral area. There were no significant differences in the number of OMP⁺ mature ORNs between the control and CSS-treated mice (n = 6, Mann–Whitney U test). OVA, ovalbumin.