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. 2020 Feb 18;14:126. doi: 10.3389/fnins.2020.00126

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Copyright © 2020 Ueha, Ueha, Kondo, Nishijima and Yamasoba.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A–C) Inflammatory cell infiltration and cell division in the nasal RM were evaluated using immunohistochemical staining (brown). Tissue sections were counterstained with the nuclear dye hematoxylin (blue). Representative immunohistochemical images of neutrophils (A), CD3⁺ lymphocytes (B), and Ki67⁺ dividing cells (C) (400× magnification), and comparative charts of cell counts in each group. *P < 0.01; **P < 0.01; ****P < 0.0001 (n = 6, one-way analysis of variance). OVA, ovalbumin; CSS, cigarette smoke solution.