Fig. 3.

a Tankyrase 2 melting curves without chymotrypsination in the absence (black) and presence (red) of a stabilizing fragment. b Tankyrase 2 melting curves treated with chymotrypsin in the absence (black) and presence (red) of the same stabilizing fragment. c Concentration-dependent response for the stabilizing fragment with chymotrypsin-digested tankyrase. d The workflow of the final lead compound optimization from the initial hit to the end was guided by DSF. This figure was adapted with permission from Larsson et al. (2013). Copyright 2013 American Chemical Society