Figure 1.
ASCs promote tumor initiation, growth and metastasis. 4T1 cells (103 cells/mouse) were injected into BALB/c female mice with or without vASCs or ASC.B6 (105 cells/mouse), control mice were injected with ASCs without tumor cells. n = 10–12. (A) Tumor initiation was regularly monitored and tumor incidence was evaluated using Kaplan-Meier analysis. (B) Tumor size was measured with a special caliper and its volume was determined as described in Material and Methods. (C) Mice were sacrificed on day 28 after tumor injection, and then primary tumors were resected and weighed. (D) The macroscopic metastatic nodules (white arrows) were counted on isolated lungs. The statistical analysis was t-test with P-values set at: *P < 0.05, **P < 0.01.