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. 2019 Dec 18;66(1):67–73. doi: 10.1262/jrd.2019-043

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©2020 Society for Reproduction and Development

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 2. — Activation and full term development of mouse oocytes injected with mouse PLCζ (mPLCζ) or equine PLCζ (ePLCζ) mRNA. (A–C) Morphology and resumption of meiosis in mPLCζ or ePLCζ mRNA-injected oocytes. Oocytes were observed every 5 min from 115 min to 135 min or 140 to 160 min, respectively. (A) Morphology of oocytes. (B, C) Rates of each stage of meiosis II in mPLCζ or ePLCζ mRNA-injected oocytes, respectively. MII, metaphase II; A/TII, anaphase /telophase II; PB, extrusion of second polar body. (D, E) Activation and pronuclear formation of zygote fertilized with inactivated spermatozoa. The oocytes were activated by mPLCζ or ePLCζ mRNA injection or SrCl₂ treatment. In the control, intact oocytes were injected with intact or inactivated spermatozoa. (F, G) Immunostaining of blastocysts activated with mPLCζ or ePLCζ mRNA injection. (F) CDX2+ cells (trophectoderm; TE) are shown in red, and Nanog+ cells (inner cell mass; ICM) are in green. (G) The average number of TE and ICM in blastocysts. (H) Development of embryo to blastocyst and full-term. The embryos were activated by mPLCζ or ePLCζ mRNA injection and fertilized with inactivated spermatozoa.