Table 1. Preimplantation development of embryos fertilized with NaOH-treated inactive spermatozoa and activated by mouse PLCζ (mPLCζ) or equine PLCζ (ePLCζ) mRNA injection, or SrCl2 treatment.
| Mouse strain | Oocyte activation method | Sperm treatment | No. of fertilized embryos |
No. of 2-cell embryos at 24 h (%) |
No. of 4–8-cell embryos at 48 h (%) |
No. of morula/ blastocysts at 72 h (%) |
No. of expanded blastocysts at 96 h (%) * |
|---|---|---|---|---|---|---|---|
| ICR | – | intact | 57 | 56 (98) | 48 (84) | 47 (82) | 37 (65) a |
| mPLCζ | NaOH | 84 | 73 (87) | 68 (81) | 66 (79) | 43 (51) | |
| ePLCζ | NaOH | 60 | 58 (97) | 55 (92) | 53 (88) | 32 (53) | |
| SrCl2 | NaOH | 85 | 81 (95) | 65 (76) | 61 (72) | 34 (40) b | |
| BDF1 | – | intact | 26 | 25 (96) | 24 (92) | 24 (92) | 19 (73) a |
| mPLCζ | NaOH | 52 | 45 (87) | 37 (71) | 36 (69) | 25 (48) b | |
| ePLCζ | NaOH | 48 | 43 (90) | 38 (79) | 36 (75) | 25 (52) b | |
| SrCl2 | NaOH | 32 | 25 (78) | 24 (75) | 24 (75) | 14 (44) b | |
* a. vs. b; P < 0.05.