. 2019 Dec 18;66(1):67–73. doi: 10.1262/jrd.2019-043

Table 2. Full term development of embryos fertilized with NaOH treated inactive spermatozoa activated by mouse PLCζ (mPLCζ) or equine PLCζ (ePLCζ) mRNA injection, or SrCl₂ treatment.

Oocyte activation method	Sperm treatment	No. of fertilized embryos	No. (%) of 2-cell embryos at 24 h	No. of transferred embryos	No. (%) of offspring *
–	Intact	184	176 (96)	165	76 (46) ^a
mPLCζ	NaOH	217	156 (72)	116	43 (37) ^a
ePLCζ	NaOH	204	187 (92)	173	41 (24) ^b
SrCl₂	NaOH	145	119 (82)	114	46 (40) ^a

* a. vs. b; P < 0.05.

Table 2. Full term development of embryos fertilized with NaOH treated inactive spermatozoa activated by mouse PLCζ (mPLCζ) or equine PLCζ (ePLCζ) mRNA injection, or SrCl2 treatment.