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Primers and restriction endonucleases used for SNPs genotyping
| ABCB1 SNP C3435T | ||||
| Forward primer (5′-3′) | Reverse primer (5′-3′) | PCR product length (bp) |
Restriction enzyme |
Genotypes (bp) |
| TTGATGGCAAAGAAATAAAGC | CTTACATTAGGCAGTGACTCG | 207 bp |
MboI (NEB: R0147L) |
(CC) 62,145 (CT) 62,145,207 (TT) 207 |
| ABCB1 SNP T129C | ||||
| TTTCACTACTTGCCCTTTCTAGAG | CGGCCTCTGCTTCTTTGAG | 258 bp |
MspA1I (NEB: R0577L) |
(TT) 258 (TC) 36,226,258 (CC) 32,226 |
