Table 1.

Sequences of the primers used to amplify the msp 1 and msp 2 genes of P. falciparum isolates

Amplification/gene	Primers	Primer sequence
Primary PCR
msp1	M1-OR	5′-CTA GAA GCT TTA GAA GAT GCA GTA TTG-3′
	M1-OF	5′-CTT AAA TAG TAT TCT AAT TCA AGT GGA TCA-3′
msp2	M2R	5′-ATG AAG GTA ATT AAA ACA TTG TCT ATT ATA-3′
	M2F	5′-CTT TGT TAC CAT CGG TAC ATT CTT-3′
Secondary PCR
K1	M1K1R	5′-AAA TGA AGA AGA AAT TAC TAC AAA AGG TGC-3′
	M1K1F	5′-GCT TGC ATC AGC TGG AGG GCT TGC ACC AGA-3′
MAD20	M1MAD20R	5′-AAA TGA AGG AAC AAG TGG AAC AGC TGT TAC-3′
	M1MAD20F	5′-ATC TGA AGG ATT TGT ACG TCT TGA ATT ACC-3′
RO33	M1RO33R	5′-TAAAGG ATG GAG CAA ATA CTC AAG TTG TTG-3′
	M1RO33F	5′-CAT CTG AAG GAT TTG CAG CAC CTG GAG ATC-3′
IC3D7	M2ICR	5′-AATACT AAG AGT GTA GGT GCA TATGCT CCA-3′
	M2ICF	5′-TTT TAT TTG GTG CAT TGC CAG AAC TTG AAC-3′
FC27	M2FCR	5′-AGAAGT ATG GCA GAA AGT AAC CCT TCT ACT-3′
	M2FCF	5′-GAT TGT AAT TCG GGG GAT TCA GTT TGT TCG-3′