Figure 2.

Chlamydia trachomatis L2 (CtL2) growth inhibition by ATC-induced Sp-dCas9 expression. (A) Microscopic images showing reduced mKate expression in inclusions formed by pTRL2-Sp-dCas9 transformants of CtL2 cultured with indicated ATC concentrations. (B) ATC concentration-dependent inhibition of EB production by the transformants. Data are averages ± standard deviations of triplicate experiments. P values are of two-tailed t tests. (A, B) ATC was added at the time of inoculation. Microscopy and determination of recoverable inclusion-forming units were performed at 30 hours post-inoculation.