Figure 3.

CtL2 growth inhibition by ATC-induced Sa-dCas9 expression. (A) Microscopic images showing reduced mKate expression in inclusions formed by pTRL2-Sa-dCas9 transformants cultured with indicated ATC concentrations. (B) ATC concentration-dependent inhibition of EB production by the transformants. Data are averages ± standard deviations of triplicate experiments. P values are of two-tailed t tests. (A, B) ATC was added at the time of inoculation. Microscopy and determination of recoverable inclusion-forming units were performed at 32 hours post-inoculation.