Figure 6.

LGK974 downregulates Wnt target genes and blocks cell cycle. (a–c) Western blotting experiments were performed by treating renal cancer cells with DMSO, at different concentrations of LGK974 (786-O, 5 μM or 10 μM; ACHN, 10 μM or 15 μM) for 72 h and then proteins were extracted. The protein levels of β-catenin, cyclin D1, and c-Myc were determined via immunoblotting. (d) Real-time PCR experiments were performed by treating renal cancer cells with DMSO, at different concentrations of LGK974 (786-O, 5 μM or 10 μM; ACHN, 10 μM or 15 μM) for 72 h and then total RNA was extracted. (e, f) Cell cycle changes after treating 786-O and ACHN cells with DMSO, at different concentrations of LGK974. All data are expressed as the mean ± SEM. The experiment was repeated at least three times. Statistical significance was determined using two-tailed Student's t-test or one-way ANOVA. ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001.