Figure 8.
Involvement of MAPK in the expression of HO-1 induced by H2 gas in differentiated PC12 cells. β-NGF-differentiated PC12 cells were pretreated with U0126 (10 mM), SB203580 (20 mM), or SP600125 (10 mM) for 1 h followed by oxygen-glucose deprivation (6 h) and then reperfusion with or without H2 gas. The expression of HO-1 protein and mRNA was measured by western blotting and qPCR, respectively. (a) Western blot analysis of β-actin and HO-1 proteins in each group. (b) Protein expression of HO-1 is shown as the HO-1/β-actin ratio for each sample. (c) The mRNA expression of HO-1 was normalized with 18S to determine the relative expression ratio (log2 fold) for each group (n = 3/group; ∗∗p < 0.01).