Table 1. The primers of two overlapped fragments.
Fragment | PCR primers | Sequences | Length of primers | Products |
---|---|---|---|---|
Fragment 1 | MIAT-F1 | 5′-GGTTCGAGTGATTCTAGTGCC-3′ | 21 bp | 703 bp |
MIAT-R1 | 5′-CCCGGAGTAAGAGGACAGAA-3′ | 20 bp | ||
Fragment 2 | MIAT-F2 | 5′-GACTCCACCATAACCATGTGCA-3′ | 22 bp | 696 bp |
MIAT-R2 | 5′-CCGTTAGGGGACAAGGACACT-3′ | 21 bp |