Figure 2.

Metabolic fate of ¹³C-glucose in TAG. (A) ToF-MS profile of major TAG species in macrophages activated in medium containing unlabeled or U-¹³C glucose. A mass shift of 3 Da was observed in TAG species of macrophages activated in the presence of labeled glucose. (B) MS/MS of 52:2 TAG (m/z 876.5), a major TAG species. A mass shift of 3 Da remains after loss of fatty acid chains induced by collision fragmentation in macrophages activated in the presence of labeled glucose. (C) Fatty acid profile obtained from macrophages activated in medium containing unlabeled and U-¹³C glucose. There was no mass shift observed in fatty acids obtained from macrophages activated in medium containing U-¹³C glucose. Data is representative of three experiments.