FIGURE 1.

The efficiency of Src inhibitor PP2 was confirmed in lipopolysaccharide (LPS)-induced BV2 activation. Cultured BV2 microglia were treated with two concentrations of PP2 (2 and 20 μM) in the presence of LPS (1 μg/ml) for 24 h. (A–C) The protein levels of p-Src and Src were determined by western blot analysis. β-Actin was used as an internal loading control. Each bar represents the mean ± SEM. n = 4. ^##P < 0.01 vs. control group,**P < 0.01, and *P < 0.05 vs. LPS group. (D) The cells were stained with anti-p-Src antibody (red) and DAPI stain (scale bar: 8 μm). (E) The fluorescence intensity of the p-Src staining of was provided in a histogram. Each bar represents the mean ± SEM. n = 3. ^###P < 0.001 vs. control group, **P < 0.01 vs. LPS group.