Table 1. The primers of genes.

Name of Gene	Primers
NMAAP1	Forward 5′-CCTTCCCCTGCCCAATA-3′ Reverse 5′-TCCACGAAACCTTCCACA-3′
TNF-α	Forward 5′-ACTGAACTTCGGGGTGATCG-3′ Reverse 5′-CCACTTGGTGGTTTGCTACG-3′
IL-1β	Forward 5′-TGGACCTTCCAGGATGAGGACA-3′ Reverse 5′-GTTCATCTCGGAGCCTGTAGTG-3′
iNOS	Forward 5′-ATGGCAACATCAGGTCGG-3′ Reverse 5′-GCACAACTGGGTGAACTCC-3′
MCP-1	Forward 5′-TTAAAAACCTGGATCGGAACC-3′ Reverse 5′-GCATTAGCTTCAGATTTACGG-3′
IL-10	Forward 5′-GTTGCCAAGCCTTATCGG-3′ Reverse 5′-GCTCTTATTTTCACAGGGGAG-3′
Arg1	Forward 5′-CAGTCTGGCAGTTGGAAGC-3′ Reverse 5′-GGTTGTCAGGGGAGTGTTG-3′
TGF-β	Forward 5′-GAGGCGGTGCTCGCTTTGTA-3′ Reverse 5′-CGTTGTTGCGGTCCACCATTA-3′
GAPDH	Forward 5′-GACTTCAACAGCAACTCCCACTC-3′ Reverse 5′-TAGCCGTATTCATTGTCATACCAG-3′

p-CMV-N-Flag/NMAAP1 eukaryotic expression vector were constructed through regular methods, and RAW264.7 cells with overexpression of NMAAP1 (ON/RAW264.7) were maintained at 37 °C and 5% CO₂ in a complete medium containing DMEM (Gibco, Grand Island, New York, USA) supplemented with 10% fetal calf serum (Gibco), 100 U/ml penicillin and 100 U/ml streptomycin (Gibco). Then, the ON/ RAW264.7 cells were treated with IL-4 (25 ng/ml) for 48 h.