Table 3.
Thrombin generation assay (in‐house) parameters. Median (5th–95th percentile) values are shown for each parameter
| TGA |
Test Category |
Normal n = 22 |
Severe HA n = 30 |
Severe HA >1 IU/dL n = 11 |
Moderate HA n = 8 |
Mild HA n = 15 |
|---|---|---|---|---|---|---|
| Peak height % | PPP+CTI | 95 (66–152) | 16**## (6.4–31.5) | 24 (15–49) | 28.5 (16–50) | 32.3 (17.8–60.5) |
| PPP | 97 (64–159) | 19.6** (6.7–39.5) | 27 (10.7–46.8) | 44.4 (18.2–47.9) | 42.5 (16.6–84.1) | |
| PRP+CTI | 97 (67–133) | 28.8 (9.3–57.6) | 28.8 (20–35.8) | 30.8 (15.2–70) | 41.6 (29.4–65.2) | |
| PRP | 104 (65–134) | 31.7 (9.5–56.9) | 31.7 (22.1–34.3) | 36.3 (24.9–65.4) | 53.5 (33.1–69.7) | |
|
Area under the thrombin generation curve % |
PPP+CTI | 90.5 (78–133) | 34.4 (12.1–56.7) | 46 (29–78) | 40 (32–70) | 53.3 (27.1–90.8) |
| PPP | 100 (72–124) | 38.8 (14.6–61.5) | 51.5 (23.2–74) | 66.5 (33.6–81.7) | 59.1 (27.9–119.5) | |
| PRP+CTI | 103 (71–115) | 37.2 (10.0–71.4) | 29.7 (22.9–42.5) | 39.7 (21.3–64.4) | 49.4 (28.6–83.8) | |
| PRP | 93 (86–114) | 39.7 (9.0–77.3) | 39.3 (21.3–46.2) | 54.7 (35.0–89.2) | 76.9 (52.0–93.7) | |
|
Time to peak thrombin generation minutes |
PPP+CTI | 12 (9–15) | 20.5 (14.4–52.6) | 18.5 (11–51) | 18.3 (11.5–28) | 17.5 (13–22) |
| PPP | 12 (9–15.5) | 20 (13.5–54.5) | 18.3 (11–39) | 15.3 (11.5–17) | 14.5 (9.5–20) | |
| PRP+CTI | 29 (20.5–49) | 50.8 (37–61) | 58.5 (53–60) | 47.5 (23.5–58) | 38.5 (32.5–56) | |
| PRP | 22 (15–41) | 50 (34.5–60) | 58.5 (54–60) | 41 (25–47.5) | 32.5 (24.5–41) |
The median and the range (5th–95th percentiles) for in‐house TGA were calculated in control group and Hemophilia patients in 4 categories. Significant difference (P value) is shown between mild HA and normal groups only. **P < .01 vs moderate; ## P < .01 vs severe >1 IU/dL.
Abbreviations: CTI, corn trypsin inhibitor; HA, hemophilia A; PPP, platelet poor plasma; PRP, platelet rich plasma; TGA, thrombin generation assay.