Figure 1.

Expression of BET family members in AtT20 cells. The mRNA expression of the BET family members Brd2, Brd3 and Brd4 was examined in AtT20 cells by qRT-PCR (A). Data from four cell passages are represented relative to Brd2 expression; ***P < 0.0005. Western blot analysis was used to confirm expression of BRD2, BRD3 and BRD4 proteins (B), with representative data from four cell passages shown; H727 cell lysate was used as a positive control, and calnexin (a housekeeper gene) was used as a loading control.