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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2019 Sep 28;195:105484. doi: 10.1016/j.jsbmb.2019.105484

Figure 2. Short-term effects of 25(OH)D on mitochondrial membrane potential and oxidation in benign prostate epithelial cells.

Figure 2.

Representative images and quantification of fluorescence from mitohealth assay on 957E/hTERT cells A-B and PrE cells C-D. Cells treated with 50nM 25(OH)D or vehicle control for 1 hour or 16 hours and mitochondrial membrane potential (red) was quantified using the 40x objective on the Cellinsight Cx7 A-D. Graphs show the average intensity in mitochondrial fluorescence (B and D). CellROX staining (orange) and quantification of 957E/hTERT cells treated with 50nM 25(OH)D, vehicle, or 5mM NAC and exposed for 1 hour or 16 hours. Graphs show mean of N=3 biological replicates (3 technical replicates within each experiment) with standard error. Student’s t-test: P<.05=* P<.005= ** P<.0005= ***.