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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2019 Sep 28;195:105484. doi: 10.1016/j.jsbmb.2019.105484

Figure 3. CYP27B1-dependent changes in mitochondrial respiration observed after 1 hour treatment with 25(OH)D.

Figure 3.

A, Transfection of 957E/hTERT cells with an siRNA for CYP27B1 induced a 50% knockdown in CYP27B1 protein levels. B-C, Seahorse XFe24 output of OCR over time from 957E/hTERT cells with a knockdown of CYP27B1. OCR tracings: si27B1 (green) and scramble (blue) treated for 1 hour with 25(OH)D relative to si27B1 (orange) and scramble (black) treated with vehicle control for 1 hour. Bar graphs show mean (±SEM) change in basal respiration and ATP production, C. D-E, Representative images and quantification of fluorescence from mitohealth assay on siCYP27B1 or scramble cells treated with 50nM 25(OH)D for 1 hour and mitochondrial membrane potential (red) was quantified. Graphs show mean of N=3 biological replicates (3 technical replicates within each experiment) with standard error. Student’s t-test: P<.05=* P<.005= ** P<.0005= ***.