Table 2. Presteady-State Kinetic Parameters of NADH-Mediated AzoA Reduction.
| wild type | W60A | |
|---|---|---|
| kred(NADH)1 (s–1) | 905 ± 35 | 472 ± 14 |
| kred(NADH)2 (s–1) | 82 ± 6 | 2.3 ± 0.3 |
| Kd(NADH)1 (μM) | 48 ± 9 | 54 ± 7 |
| Kd(NADH)2 (μM) | 187 ± 38 | 75 ± 32 |
| Δa1/Δa2 | 93/7 | 79/21 |
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