Figure 4.

TRIM31 is a direct target gene of miR-29c-3p. (A) TargetScan and miRanda were used to predict the 3′-UTR binding sites of miR-29c-3p and TRIM31. (B) The luciferase activity of wild-type TRIM31-3′UTR (wt) and mutant TRIM31-3′UTR (mut) in HCC cells overexpressing miR-29c-3p in liver cancer cells (MHCC-97H and HepG2). (C and D) qRT-PCR and western blotting were used to analyze the mRNA and protein levels of TRIM31 in MHCC-97H cells that were co-transfected with miR-29c-3p mimics and miR-29c-3p inhibitor. *P<0.05 compared to the control. TRIM31, tripartite motif containing 31.